Making AppleTalk inactive (either in the Chooser or the AppleTalk control panel) is not sufficient, since the resources are loaded at startup.
Follow these steps to make AppleTalk inactive under Classic Networking:
2. Click the Inactive button to make AppleTalk inactive.
3. Restart the computer.
For additional information on how to turn off AppleTalk under Open Transport, please see article 24346: "Mac OS: AppleTalk "Port in Use" Error"
Additional Workaround
Install Apple Remote Access Client, which adds the option "Remote Only" to the AppleTalk Control panel. Choosing "Remote Only" routes AppleTalk to "Remote Only", freeing up your printer port for use by your serial printer.