Yes, you should install HSU 2.0.1 over System 7 Pro.
The Apple Installer Script, version 1.0, REMOVES HSU if it's installed, and
doesn't replace it with a new copy. Installer Script 1.0.1 fixes this
problem. There are approximately 18,000 System 7 Pro copies that have
Installer Script 1.0. If you suspect that HSU was removed from a system,
check the Extensions folder for Hardware System Update. If it isn't there,
install HSU 2.0.1 again.
The HSU that Apple Installer Script 1.0.1 installs is exactly the same as the
one the Hardware System Update disk installs, except the creation date is
earlier.
If you install System 7 Pro with Custom Install on a system that already has
HSU 2.0.1, you'll see a dialog box which says that the existing version is
newer and asking whether you want to replace it. Since the HSU files are also
found in System 7 Pro and are the same except for creation date, you can
either replace or leave the existing file.
Support Information Services