The DOS Compatibility Card joystick port should not be removed. While the joystick card is a separate from the DOS Compatibility Card daughter board, the joystick card is how the DOS Compatibility Card accesses the PDS slot. The DOS Compatibility Card daughter board has a 26-pin ribbon cable connecting to the joystick card. If you remove the joystick card, the DOS Compatibility Card no longer functions, and a message appears on the screen with the message "The DOS Compatibility Card is not installed. Changes made will take effect when the card is installed."